Thermo / Applied Biosystems Prism 377 DNA Sequencer
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Thermo / Applied Biosystems Prism 377 DNA Sequencer

Features

  • Gel Electrophoresis
  • Sequencing and GeneScan Applications
  • Manual and Automatic Processing

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This Applied Biosystems Prism 377 DNA Sequencer is used and in excellent condition. This is the 377 DNA Sequencer only and does not include the computer, software, or accessories. Please contact the manufacturer for pricing and availability.

Power Input: 200-240 VAC, Single Phase

Dimensions (Unit Only): 29" W x 20" D x 32" H (73.66cm W x 50.8cm D x 81.28cm H)

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View Photo of this unit with the front door open
View Photo of this unit's catch bottle
View Photo of this unit's cooling valve



Stock # 62537-2




This Bel Fuse / Power-One NRG3D15A8D4K0FZ107 is the Thermo / Applied Biosystems Prism 377 DNA Sequencer Power Supply.

Part Number: LR38879, 073-62624-101, 073-61162-106, 073-61090-104



Stock # 62537-18




This Fasco U62B1 is the Thermo / Applied Biosystems Prism 377 DNA Sequencer Motor W/ Pump Assembly.

Part Number: K10136, 7162-3335



Stock # 62537-7




This Grainger / Dayton Electric 2C915A is the Thermo / Applied Biosystems Prism 377 DNA Sequencer Shaded Pole Blower.

Part Number: 1TDP6



Stock # 62537-17




This Lytron / Lydall / Affinity 6110G1SB is the Thermo / Applied Biosystems Prism 377 DNA Sequencer Heat Exchanger.



Stock # 62537-13




This Perkin Elmer / Applied Biosystems 683327 is the Thermo / Applied Biosystems Prism 377 DNA Sequencer Motherboard.



Stock # 62537-12




This Potter & Brumfield CNS-35-92 is the Thermo / Applied Biosystems Prism 377 DNA Sequencer Programmable Time Delay Relay.



Stock # 62537-19




This Potter & Brumfield P25P42A22P1-240 is the Thermo / Applied Biosystems Prism 377 DNA Sequencer Contactor.



Stock # 62537-21




This SAE Power GA5B-30 is the Thermo / Applied Biosystems Prism 377 DNA Sequencer Power Line Filter



Stock # 62537-20




This Spellman X2094 is the Thermo / Applied Biosystems Prism 377 DNA Sequencer High Voltage Power Supply.

Part Number: PTV5P300X2094



Stock # 62537-15




This Thermo / Applied Biosystems 005157-1 is the Prism 377 DNA Sequencer Servo Motor.

Part Number: 4241B680-R2



Stock # 62537-4




This Thermo / Applied Biosystems 005064-1 is the Prism 377 DNA Sequencer Thermostat Housing.



Stock # 62537-14




This Thermo / Applied Biosystems 603832 is the Prism 377 DNA Sequencer Heater Transfer Plate.



Stock # 62537-10




This Thermo / Applied Biosystems 603924 is the Prism 377 DNA Sequencer Power Distribution EMI Board.



Stock # 62537-22




This Thermo / Applied Biosystems is the Prism 377 DNA Sequencer CPU Board.

Part Number: 95PCB00500



Stock # 62537-8




This is the Thermo / Applied Biosystems Prism 377 DNA Sequencer CCD Detector Assembly.

Part Number: 603437, SC0564NNN-911



Stock # 62537-6




This is the Thermo / Applied Biosystems Prism 377 DNA Sequencer Heat Transfer Plate.



Stock # 62537-11




This is the Thermo / Applied Biosystems Prism 377 DNA Sequencer Optic Module.

Part Number: 527EFLP, 042/9640



Stock # 62537-5




This VIAVI Solutions / JDSU 2214-40MLA is the Thermo / Applied Biosystems Prism 377 DNA Sequencer Argon Ion Laser.



Stock # 62537-9




This VIAVI Solutions / JDSU 2114B-40MLA is the Thermo / Applied Biosystems Prism 377 DNA Sequencer Laser Power Supply.



Stock # 62537-16




This Applied Biosystems Prism 377 DNA Sequencer is used and in excellent condition. This item includes a computer controller, manual and various accessories.

Software Installed:
ABI Gene Scan v3.1
ABI Sequencing Analyzer 3.3
ABI 377-96 DNA Sequencer Data Collection v2.5

Power Mac 7200/120:
Processor: 120 MHz
OS: OS8
HDD: 1.2GB
MEMORY: 8 MB
Power Input: 100-240 VAC, Single Phase

Power Input DNA Sequencer: 200-240 VAC, Single Phase

Dimensions (DNA Sequencer Only): 29" W x 20" D x 32" H (73.66cm W x 50.8cm D x 81.28cm H)

View Photo of the computer controller with its accessories.
View Photo of the DNA sequencer accessories.

This system's hardware and software configuration is preserved, as it was originally used. As the end user, it will be the user's responsibility to comply with any non-transferable software licensing issues and delete any software that is deemed non-transferable.



Stock # 62537-3




This Applied Biosystems Prism 377 DNA Sequencer is used and in excellent condition.

This unit was decommissioned from a production facility. We do not have the expertise to test this unit. A very good effort was made to retain its components and spares together and we have documented all the information we can gather from it in this page.

Software Installed:
ABD 377 Diagnostics
Sequencing Analyzer 3.4.1
ABI Prism 377-96 Collection

Mac Computer:
Processor: 450 MHz
OS: OS9
HDD: 10GB
MEMORY: 64 MB

Power Input: 200-240 VAC, Single Phase

Dimensions (Unit Only): 29" W x 20" D x 32" H (73.66cm W x 50.8cm D x 81.28cm H)

View Photo of the computer controller.
View Photo of the first group of accessories.
View Photo of the second group of accessories.
View Photo of the third group of accessories.
View Photo of the fourth group of accessories.
View Photo of the fifth group of accessories.
View Photo of the sixth group of accessories.
View Photo of part number 401839, 36 cm plain glass plate.
View Photo of part number 401840, 36 cm notched glass plate.
View Photo of part number 4305384, 36 cm step glass plate.
View Photo of part number 401835, 48 cm plain glass plate.
View Photo of part number 401838, 48 cm notched glass plate.

This system's hardware and software configuration is preserved, as it was originally used. As the end user, it will be the user's responsibility to comply with any non-transferable software licensing issues and delete any software that is deemed non-transferable.



Stock # 62537-1



  • Description
  • Tech Specs
  • Datasheets / Manuals 
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Description

The ABI PRISM 377 DNA Sequencer is an automated instrument designed for analyzing fluorescently-labeled DNA fragments by gel electrophoresis. Gel electrophoresis is the movement of charged molecules through a gel solution (such as polyacrylamide) in an electrical field. It is used to separate DNA fragments by size.

The instrument functions the same way for both sequencing and GeneScan applications. The main differences between these applications is in the preparatory chemistries used, and the type of analysis performed on the resulting data.

There are four different models of the ABI 377:

ABI PRISM 377 DNA Sequencer

The standard ABI PRISM 377 instrument is designed to accommodate three sizes of gel plates—12 and 36 cm for GeneScan applications, and 36 and 48 cm for sequencing applications. Combs available are 18, 24, 32, 36, and 48-well shark’s tooth, and 24, 32, and 36-well square tooth. Data is collected from 194 channels during each scan.

ABI PRISM 377-18 DNA Sequencer

The ABI PRISM 377-18 DNA Sequencer is the lowest cost, lowest-throughput version of the instrument. It can run up to 18 lanes on a single gel.

ABI PRISM 377 DNA Sequencer with XL Upgrade

The ABI PRISM 377 DNA Sequencer with XL Upgrade increases the number of samples that can be analyzed simultaneously. Increased throughput is made possible by modifying the instrument to collect data from 388 channels instead of 194 during each scan. The XL Upgrade can accommodate the following combs: 48- and 64-well shark’ s tooth combs for sequencing applications; 48-, 50-, 64-, and 66-well for GeneScan applications. You can still use 36-w ell or other lo wer lane density combs if desired. Refer to the ABI PRISM® 377 DNA Sequencer XL Upgrade User’s Manual (P/N 904412) for more information.

ABI PRISM 377 DNA Sequencer with 96-Lane Upgrade

The ABI PRISM 377 DNA Sequencer with 96-lane Upgrade increases the number of samples that can be run on each gel. Increased throughput is made possible by re-engineering the instrument to collect data from 480 channels instead of 388 for the ABI PRISM 377 DNA Sequencer with XL Upgrade or 194 for the ABI PRISM 377 DNA Sequencer. In addition, the scan region has been increased from 6 inches to 7.25 inches. The 96-lane upgrade includes a 100-well shark’s tooth comb, and a new front glass plate. The new front glass plate has a bevel where samples are loaded. The bevel increases the thickness of the gel from 0.2 mm to 0.4 mm in this region. Lower lane density combs can still be used, but only with the original front glass plates provided with the standard ABI PRISM 377 instrument.

Product Family: 377, 377-18, 37718 377 18, 377-XL, 377XL, 377 XL, 377-96, 37796, 377 96
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Specifications

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Resources

Manuals, Datasheets, Drivers, Links

Applied Biosystems Prism 377 Datasheet (pdf) 
Applied Biosystems Prism 377 with 96-Lane Upgrade Datasheet (pdf) 
Applied Biosystems Prism 377 User Manual (pdf) 
Applied Biosystems Prism 377 with XL Upgrade User Manual (pdf) 
Applied Biosystems Prism 377 with 96-Lane User Manual (pdf) 
Applied Biosystems Prism 377 GeneMapper Software User Manual (pdf) 
Applied Biosystems Prism 377 SegScape Software User Manual (pdf) 
Applied Biosystems Prism 377 Chemistry Reference Manual (pdf) 
Applied Biosystems Prism 377 Achieving Better Results User Bulletin (pdf) 
Applied Biosystems Prism 377 Cleaning Glass Plates User Bulletin (pdf) 
Applied Biosystems Prism 377 Matrix Standards User Bulletin (pdf) 
Applied Biosystems Prism 377 with 96-Lane Upgrade Optimizing Resolution User Bulletin (pdf) 
Applied Biosystems Prism 377 Parts List (pdf) 
Applied Biosystems Prism 377 Full Manual (pdf) 
Link to Thermo / Applied Biosystems Website 
Link to Bel Fuse / Power-One Website 
Link to VIAVI Solutions / JDSU Website 
Link to Spellman Website 
Link to Fasco Website 
Link to Lytron / Lydall / Affinity Website 
Link to Perkin Elmer / Applied Biosystems Website 
Link to Tyco Electronics / Potter and Brumfield Website 
Link to Grainger / Dayton Electric Website 
Link to SAE Power Website