V6 GE Healthcare Interface Module

MegaBACE 1000 DNA Analysis System is a high-throughput, fluorescence-based DNA system utilizing capillary electrophoresis with up to 96 capillaries operating in parallel. The system performs sample injection, gel matrix replacement, DNA separation, detection, and data analysis, offering unparralleled flexibility.

Components •Capillary Electrophoresis Instrument
•Up to 6 Arrays of 16 Capillaries/Array for a Total of 96 Capillaries
•Separation Matrix for DNA Analysis
•Instrument Control and Data Analysis Software
•Computer Workstation for Instrument Operation and Data Analysis

Capillary Arrays The capillaries are supplied in pre-assembled arrays of 16 capillaries. 16, 32, 48, 64, 80 or 96 capillaries can be used during a run.
•200 µ Outer Diameter
•75 µ Internal Diameter
•40 cm Detection Length

Sequencing Read Length and Throughput With the ability to sequence 96 samples in just 2 hours and perform up to 9 runs per day, MegaBACE 1000 is the ideal choice for production-scale sequencing projects. Average read length with dye primer chemistry is 550 bp at 98.5% accuracy with M 13 standard template. With a slower, 3-hour run time, sequences of up to 1000 bases is possible. MegaBACE 1000 can produce more than 500 samples per 9-hour day.

Sample Requirement Minimal sample loading volume of 5 µl. The samples are introduced to the system in a 96-well format. Samples can be reinjected up to 10 times.

Excitation
•Single Mode (488 nm) for Sequencing
•Dual mode (488 and 532 nm) for Genotyping Detection

4 Channels with 2 PMTs
•Confocal Optics with Multiple Filter Sets
•Digital Resolution is 16-bits Linear to 5%