NEP3229-002 Barocycler Pressure BioSciences Pressure Cycling Technology Sample Preparation System (PCT SPS)

The Pressure BioSciences NEP3229 Barocycler Pressure Cycling Technology Sample Preparation System (PCT SPS) is an affordable, high pressure laboratory instrument designed to fit on a bench top, inside a biological safety cabinet, or on the shelf of a cold room. The Barocycler NEP3229 is capable of processing up to three samples simultaneously using specially designed, single-use containers called PULSE Tubes. The Barocycler NEP3229 has an external chiller hook-up, automatic fill and dispense valves, and an integrated micro-processor with an easy-to-use keypad. The new bench top Barocycler NEP3229 fills an important and growing need in genomics, proteomics, and metabolomics for the rapid, robust, versatile, reproducible, and quantitative extraction of nucleic acids, proteins, and small molecules from a wide variety of organisms including, viruses, bacteria, plant and animal cells and tissues.

PULSE (Pressure Used to Lyse Samples for Extraction) Tubes (PT) transmit the power of PCT from the Barocycler instrument to the sample. For bio-molecule extraction, the specimen (such as tissue) is placed on the Lysis Disk, the PT is placed in the pressure chamber of the Barocycler, pressure chamber fluid is added, and pressurization begins. As pressure increases, the Ram pushes the specimen from the Sample Chamber through the Lysis Disk and into the Fluid Retention Chamber. When pressure is released, the sample (now partially homogenized) is pulled back through the Lysis Disk by the receding Ram. The combination of physical passage through the Lysis Disk, rapid pressure changes, and other biophysical mechanisms breaks up the cellular structures of the specimen to quickly and efficiently release nucleic acids, proteins, and small molecules.